منابع مشابه
Enzyme catalysed tandem reactions.
To transfer to the laboratory, the excellent efficiency shown by enzymes in Nature, biocatalysis, had to mimic several synthetic strategies used by the living organisms. Biosynthetic pathways are examples of tandem catalysis and may be assimilated in the biocatalysis field for the use of isolated multi-enzyme systems in the homogeneous phase. The concurrent action of several enzymes that work s...
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Since the pH is treated as an independent variable in biochemical thermodynamics, the Gibbs energy G does not provide the criterion for equilibrium, but the transformed Gibbs energy G’ does. The standard transformed Gibbs energy of formation DfG’ of a reactant (sum of species) can be calculated at the desired temperature, pH, and ionic strength if the standard Gibbs energies of formation DfG an...
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In this paper the nature and validity of the mathematical formulation of MichaelisMenten type kinetics for enzyme-catalysed biochemical reactions is studied. Previous work has in the main concentrated on isolated, spatially uniform (well-mixed) reactions. We investigate the effects of substrate input and diffusion on this formulation, in particular, on the nature and validity of the quasi-stead...
متن کاملA practical guide to modelling enzyme-catalysed reactions.
Molecular modelling and simulation methods are increasingly at the forefront of elucidating mechanisms of enzyme-catalysed reactions, and shedding light on the determinants of specificity and efficiency of catalysis. These methods have the potential to assist in drug discovery and the design of novel protein catalysts. This Tutorial Review highlights some of the most widely used modelling metho...
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Combined quantum mechanics/molecular mechanics (QM/MM) modelling has the potential to answer fundamental questions about enzyme mechanisms and catalysis. Calculations using QM/MM methods can now predict barriers for enzyme-catalysed reactions with unprecedented, near chemical accuracy, i.e. to within 1 kcal/mol in the best cases. Quantitative predictions from first-principles calculations were ...
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ژورنال
عنوان ژورنال: Current Opinion in Chemical Biology
سال: 2013
ISSN: 1367-5931
DOI: 10.1016/j.cbpa.2013.02.015